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Fiber Photometry for non-RZ10 Processors

Common Use Cases

Real-time control and acquisition of demodulated locked-in amplification signal from any combination of up to 4 light drivers and 2 photosensors. This is the primary gizmo used in fiber photometry setups using processors other than an RZ10 or RZ10x. Record up to 8 demodulated signals with raw photosensor output too.

Data Stored
Stream Demodulated response signals
Stream (optional) Broadband raw signals
Scaler (optional) Driver parameters
Outputs
Driver output voltage (optional) Up to 4 single-channel floats
Calculated signals (optional) Up to 4 single-channel floats

Gizmo Help Slides

Reference

The Fiber Photometry gizmo, when used with processors that are not the RZ10, includes designtime and runtime control of up to four voltage output signals to drive external light drivers. It uses lock-in amplification to measure the real- time power of the resulting fluorescence response at the light driving frequencies. It controls up to four light driver signals and reads two photosensors via the RZ analog inputs.

The Runtime Interface

Runtime Plot

A plot is added to the runtime window for visualization.

Flow Plot Showing Demodulated Responses

The subplots shown in a runtime plot represent data storage you chose in the designtime options. In the example above, the streamed data shows the resulting power output (such as Dv1A) at the frequency of interest when comparing the selected driver (such as Drv1) to the selected sensor input (such as sensor A). Simultaneous neural recordings from a different gizmo are integrated in the plot for a quick visual comparison. The Fiber Photometry gizmo also stores and displays broadband raw input signals and driver parameters, depending on selections made at designtime.

Runtime Controls

Runtime Interface

The runtime window includes:

Options Description
Clipping Indicator(s) Two indicators, one for each sensor, flash when the user-defined clipping threshold is crossed. The clipping indicator will also light up if the input voltage is below 10uV to indicate a bad connection.
Lowpass Filter A knob and value entry box that controls the lowpass filter that determines the bandwidth around each demodulation frequency used for the power calculation.
Dv{x} Options
Light On A button enables the light driver and an indicator is lit green when the light is on.
Parameters Knobs and value entry boxes allow runtime control of light driver Frequency, Level, and DC Offset parameter values.
Results A result for each sensor is dynamically displayed as a single value in millivolts.

Fiber Photometry Configuration Options

See The Options Area and Templates for more information on the gizmo name, source, global options, and displaying the block diagram.

Driver(s) Tab

Driver(s) Tab

Drv-{n} Options Description
Name Three characters that serve as the first three characters in the store name.
Output Specify the DAC number (channel number in parenthesis), or send this as a gizmo output.
Cal Factor Scale factor used to convert desired milliamps into voltage.
Defaults Options
Frequency Modulation frequency of the light source sine wave.
Level Peak-to-peak light source sine wave amplitude.
Offset DC shift added to the output sine wave. The offset is adjusted to reach the linear range of the physical light driver output and minimize signal distortion. More on that later.

Sensor(s) Tab

Sensor(s) Tab

Sensor Option Description
Name Give the sensor a name as it will appear in the runtime interface. The first letter of the sensor name is used as the last letter of the streaming data store name.
Source The sensor input can be an analog input from the front panel of the hardware or a floating point signal output of another hardware source or gizmo.
Calibration Factor Scales the sensor data.
Clip Threshold Raw A/D sensor input voltage value to light runtime indicator (no calibration factor applied).
Sensor B check box Enables a second sensor input.
Demodulator Option
Filter Order Higher order filters tighten the band around the response frequency.
Default Lowpass Frequency Determines the band around the frequency of interest to do the demodulation calculation. This can be modified at runtime.

Demodulator(s) Tab

Demodulator(s) Tab

Demodulator Options (Saved at 1K Rate)

Use the matrix of check boxes to select the combinations of sensors and drivers that will be used for demodulation. All available sensor signals can be demodulated against all light driver signals if desired. These data store at 1kHz sampling rate.

Calculated Outputs

Perform up to four real-time calculations on the demodulated data streams. Choose a Source demodulated signal, optionally subtract another demodulated signal in the Difference with... column, and optionally perform a delta F over F calculation on it (dF/F checkbox) to compare the strength of the signal relative to the baseline.

Saving (1K Rate)

The calculated signal is available as a gizmo output so other gizmos can attach to it and do further processing in real-time. The calculated signal can also be optionally shown on the Flow Plot at runtime, and optionally be saved to disk (at 1kHz sampling rate) as well.

df/F Options - Default Window Duration

Sliding average window size used as the baseline for the delta F over F computation.

Misc Tab

Misc Tab

Required Sample Rate

Tells the RZ what minimum sample rate this gizmo requires. Typically 6K is enough. Only increase this if the driver frequency needs to go beyond 1-2kHz for your experiment, which is rarely done.

Drivers On at Runtime

When selected, output driving signals are "on" when recording begins. Otherwise, light drivers must be turned on manually in the runtime interface.

Store Driver Signals

Stores the signals used to drive the LEDs at the RZ system rate. The store name is the first two letters of the gizmo name, followed by the last letter of gizmo name, followed by 'd' (default 'Fi1d'). One channel per light driver.

Store Driver Parameters

All light driver parameters are timestamped and stored to disk two seconds after a change has been made to any of the driver parameters in the runtime user interface.

Store Sensor Signals

Stores the raw sensor signals at the RZ system rate. The store name is the first two letters of the gizmo name, followed by the last letter of gizmo name, followed by 'r' (default 'Fi1r'). One channel per sensor.