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Fiber Photometry System

Conduct calcium imaging experiments with our fiber photometry processor and Synapse software.

The TDT Fiber Photometry System features the RZ5P acquisition processor and our flagship Synapse software. Designed specifically to fit the growing needs of customers who wanted to perform quality calcium imaging and optogenetics experiments with access to electrophysiology and control of external behavioral devices.

 

Our software and hardware allow for seamless integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with no delay from our real time processor. Correlate neural responses to behavioral states as well as external stimuli such as sound, sight and smell.

 

Fiber Photometry Experiments have never been so easy to setup and simultaneously run with other research projects. Learn more about the Fiber Photometry Setup.

Complete control for simultaneously collect data from multiple awake, behaving animals or multiple sites on the same animal
Complete control for simultaneously collect data from multiple awake, behaving animals or multiple sites on the same animal
For a Custom System, Contact TDT Sales Today!

Photometry System Components:

(1) RZ5P Fiber Photometry  Processor

Process and filter acquired signals in real-time.

(1) Synapse Software Suite

Freely control and manipulate your data with our user extensible software.

How easy it is to use the TDT system for Fiber Photometry?

Request a Fiber Photometry Live Demo

Finally – a high performance processor with maximum flexibility.

Having been in production for several years, the RZ5P has been proven as an effective hardware platform for Electrophysiology, Optogenetics, and Fiber Photometry. Our customers are always pushing the cutting-edge of science, and the RZ5P is a model example of how TDT and our System 3 platform grows with our customer base.

 

Our R&D team collaborated closely with leading labs in fiber photometry, including exceptional assistance from Dr. Thomas J. Davidson at the University of California San Francisco. Consequently, the RZ5P was designed to deliver the highest quality photometric data with a focus on ease of use.

Fiber Photometry research simultaneous acquisition with Optogenetics and Electrophysiology

Specs: RZ5P Fiber Photometry Processor

  • 4 Channels of DAC for controlling multiple LEDs (+/- 10V)
  • 4 Channels of ADC for multiple photoreceivers and other analog signals (+/- 10V)
  • 24 Channels of Digital I/O for 3.3V or 5V digital communication

User Manual | RZ5P Processor>

Multi-Subject, Multi-Site, Multi-Signal

Independent GCaMP control of up to four animals using multiple fiber optics sent to multiple subjects.
Use the same fiber patch cord to simultaneously record from Multiple Sites
Easy integration of electrophysiology and other signals to your current signal recordings.

Record from Multiple Subjects

 

The flexibility of our fiber photometry system means that multiple fiber optics can be sent to multiple subjects. Have independent GCaMP + isosbestic control of up to two animals, or independent GCaMP control up to four animals.

Record from Multiple Sites

 

Send multiple single or branching fiber patch cords to different sites for dissecting the dynamics of neural circuitry on a wider scale. You have independent control of up to four LED sources with the RZ5P DAC outputs.

Record Other Neurophysiological Signals

 

The RZ5P + Synapse is a flexible platform that allows for easy integration of electrophysiology and other signals to your current experiment and recordings. The RZ5P comes pre-built with the capability to add on a PZ5 amplifier for doing up to 32 channels of ephys. Other analog signals can be captured by the remaining ADC inputs of the RZ5P.

Lock-In Amplification

See many clear demodulated fluorophore responses in real time.

Our fiber photometry system works off the principle of locked-in amplification, wherein LED lightsources are driven at high frequencies (200 - 500 Hz) and low-frequency fluorescent responses are demodulated out of the raw photostream.

Technique

 

Lock-in amplification is a signal processing technique that uses modulation of driver signals and an orthogonal reference signal (cosine of the driver signal) to extract relevant amplitude and phase of frequency-specific responses in a complex and often noisy signal. We drive multiple signals at different frequencies and then extract only the contribution from each of those frequencies in the acquired photodetector signal – that’s how we can detect multiple fluorescent simultaneously off of a single photosource.

Benefits

 

Lock-in amplification has many benefits. First, it allows for high-fidelity signal detection in noisy environments. Since signals are extracted based on their frequency content, the fluorescent signal is not affected by parasitic room lighting or electronic noise. Additionally, this technique allows for detecting several signals simultaneously off the same fiber optic cable (405 isobestic, 465 GCaMP, 560 TDtomato, etc).

Difference

 

Other systems use CMOS cameras or light spectroscopy to pick up fluorescent signal responses. This normally requires multiple fibers per light source and is a generally noisier technique. Our lock-in amplification makes it easy to send multiple light sources down the same fiber to detect several independent signals from the same site. No need for multiple fibers - one site, several colors, clean signal!

Fiber Photometry with Lock-in Amplification for detecting several fluorescent signals simultaneously

Fiber Photometry Experiment Gizmos

Fiber Photometry Gizmo >

TDT Engineers brought the RZ5P Fiber Photometry Processor from concept to production and worked with our technical support team to ensure the Fiber Photometry Gizmo is relatively easy to use for any lab.

 

Even the novice at Fiber Photometry experiments can master the technique relatively easily.  With the Drop and Go Intuitive Gizmo functionality, the Synapse software allows  you to easily connect other devices and directly control all of your S3 Devices.

Fiber Photometry Setup:

Step One: Connect

Step Two: Drop & Go Gizmo

Step Three: Press Record

Connect & Turn On

  • Connect Sensors & Drivers and turn on RZ5P Fiber Photometry Processor with Synapse Software.

 

TDT Fiber Photometry (RZ5P) processor controls the voltage signal for the LED light driver, captures the photoreceiver response, and performs online real-time demodulation.

Connect the LED driver and light sources to the RZ5P outputs and the photoreceiver to the RZ5P input with BNC cables, and fiber-optic patch cords between all the light I/O modules.

Easily connect your Fiber Photometry LED Driver, Light sources, flouorescence ports and photo receiver to the RZ5P Processor.

Drop & Go Synapse Gizmo

  • To make your life easier, Fiber Photometry has been encapsulated in a single gizmo, found under the neural group in the gizmo list.

 

Synapse software controls the signal parameters on the RZ5P and reads the data for viewing and storage.

The RZ5 platform features two or three ultra fast digital signal processors for optimal perfomance. Since Synapse will be handling your digital signal processor (DSP) details, like assigning tasks to the DSP and distributing tasks evenly, you only need to worry about higher level functions like organizing multiple experiments across numerous subjects and various lab members.

Synapse Software Screenshot of the Fiber Photometry Gizmo controls.

Press Record

  • Just like that, begin collecting and visualizing real-time data on fiber photometric responses.

 

Now, easily integrate video recording, behavioral study, electrophysiology recordings, and optogenetic stimulation. Any combination of these modalities can be run simultaneously, and with these results you can further bolster your hypotheses for your research.

TDT's Synapse Software Gizmo screen real-time  collecting and visualizingdata on fiber photometric responses.

Our TDT Support experts can walk you through different configurations for Fiber Photometry as well as answer your questions via a personalized live demo.

Request to Talk to one of our experts now

Fiber photometry analysis using techniques such as data smoothing, bleach detrending, and z-score analysis.

Fiber Photometry Epoch Averaging Example:

https://www.tdt.com/support/EXEpocAveragingExampleDR.html

Data Import and Analysis

Extract the photometry signal and analyze the fluorescence output.

Easily import our data, both while Synapse is recording and for post-processing, or write yourown custom Matlab code.

With TDT’s new MatlabSDK, your data will be imported as a structure for straightforward handling of all streams, snips, and epoc events. There are many optional functions in the SDK, such as TDTfilter, which lets you filter data around epoc events to make peri-event response plots. You can also look at our Offline Analysis Matlab Workbooks, which are full-code examples of how to import data into Matlab and do interesting things.

 

There are also optional exports to ascii or other formats for analysis in Excel.

Commitment to Research

Get a quote Started today  >

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For a Custom System, Contact TDT Sales Today!
Independent GCaMP control of up to four animals using multiple fiber optics sent to multiple subjects.
Fiber Photometry with Lock-in Amplification for detecting several fluorescent signals simultaneously
Easily import our data, both while Synapse is recording and for post-processing, or write yourown custom Matlab code.