The TDT Fiber Photometry System features the RZ5P acquisition processor and our flagship Synapse software. Designed specifically to fit the growing needs of customers who wanted to perform quality calcium imaging and optogenetics experiments with access to electrophysiology and control of external behavioral devices.
Our software and hardware allow for seamless integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with no delay from our real time processor. Correlate neural responses to behavioral states as well as external stimuli such as sound, sight and smell.
Fiber Photometry Experiments have never been so easy to setup and simultaneously run with other research projects. Learn more about the Fiber Photometry Setup.
Process and filter acquired signals in real-time.
Freely control and manipulate your data with our user extensible software.
Having been in production for several years, the RZ5P has been proven as an effective hardware platform for Electrophysiology, Optogenetics, and Fiber Photometry. Our customers are always pushing the cutting-edge of science, and the RZ5P is a model example of how TDT and our System 3 platform grows with our customer base.
Our R&D team collaborated closely with leading labs in fiber photometry, including exceptional assistance from Dr. Thomas J. Davidson at the University of California San Francisco. Consequently, the RZ5P was designed to deliver the highest quality photometric data with a focus on ease of use.
Specs: RZ5P Fiber Photometry Processor
User Manual | RZ5P Processor>
Record from Multiple Subjects
The flexibility of our fiber photometry system means that multiple fiber optics can be sent to multiple subjects. Have independent GCaMP + isosbestic control of up to two animals, or independent GCaMP control up to four animals.
Record from Multiple Sites
Send multiple single or branching fiber patch cords to different sites for dissecting the dynamics of neural circuitry on a wider scale. You have independent control of up to four LED sources with the RZ5P DAC outputs.
Record Other Neurophysiological Signals
The RZ5P + Synapse is a flexible platform that allows for easy integration of electrophysiology and other signals to your current experiment and recordings. The RZ5P comes pre-built with the capability to add on a PZ5 amplifier for doing up to 32 channels of ephys. Other analog signals can be captured by the remaining ADC inputs of the RZ5P.
Our fiber photometry system works off the principle of locked-in amplification, wherein LED lightsources are driven at high frequencies (200 - 500 Hz) and low-frequency fluorescent responses are demodulated out of the raw photostream.
Lock-in amplification is a signal processing technique that uses modulation of driver signals and an orthogonal reference signal (cosine of the driver signal) to extract relevant amplitude and phase of frequency-specific responses in a complex and often noisy signal. We drive multiple signals at different frequencies and then extract only the contribution from each of those frequencies in the acquired photodetector signal – that’s how we can detect multiple fluorescent simultaneously off of a single photosource.
Lock-in amplification has many benefits. First, it allows for high-fidelity signal detection in noisy environments. Since signals are extracted based on their frequency content, the fluorescent signal is not affected by parasitic room lighting or electronic noise. Additionally, this technique allows for detecting several signals simultaneously off the same fiber optic cable (405 isobestic, 465 GCaMP, 560 TDtomato, etc).
Other systems use CMOS cameras or light spectroscopy to pick up fluorescent signal responses. This normally requires multiple fibers per light source and is a generally noisier technique. Our lock-in amplification makes it easy to send multiple light sources down the same fiber to detect several independent signals from the same site. No need for multiple fibers - one site, several colors, clean signal!
Fiber Photometry Gizmo >
TDT Engineers brought the RZ5P Fiber Photometry Processor from concept to production and worked with our technical support team to ensure the Fiber Photometry Gizmo is relatively easy to use for any lab.
Even the novice at Fiber Photometry experiments can master the technique relatively easily. With the Drop and Go Intuitive Gizmo functionality, the Synapse software allows you to easily connect other devices and directly control all of your S3 Devices.
Fiber Photometry Epoch Averaging Example:
With TDT’s new MatlabSDK, your data will be imported as a structure for straightforward handling of all streams, snips, and epoc events. There are many optional functions in the SDK, such as TDTfilter, which lets you filter data around epoc events to make peri-event response plots. You can also look at our Offline Analysis Matlab Workbooks, which are full-code examples of how to import data into Matlab and do interesting things.
There are also optional exports to ascii or other formats for analysis in Excel.
We understand that every experiment is dependent on your specific research. TDT service professionals work with one job in mind: to ensure that you get the help you need.
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