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Fiber Photometry System

RECORD REAL-TIME FLUOROPHORE RESPONSES WITH THE RZ10X

Description

Specs & Resources

Publications

Fiber photometry is a calcium imaging method for detecting neural activity. Researchers can use genetic animal models and fluorescent proteins, such as calcium (GCaMP) and dopamine (dLight), to study specific brain circuits.

TDT’s Fiber Photometry is an out-of-the-box solution designed for researchers. Our user-friendly Our user-friendly Synapse software provides a seamless interface to the real-time LUX RZ10X processor. Integrated LUX LEDs and photosensors streamline hardware setup and debugging. TDT allows you to focus on the research and not the equipment.

Synapse and our LUX hardware simplify fiber photometry. With Synapse, record and view your fluorophore responses in real-time, while behavior and video data is automatically time-locked. Optogenetics and electrophysiology can also be incorporated for multi-modal and closed-loop experiments.

Guo … Polley, et al “The Cholinergic Basal Forebrain Links Auditory Stimuli with Delayed Reinforcement to Support Learning”. Neuron Jul, 2019. doi: 10.1016/j.neuron.2019.06.024

Parker … Bruchas, et al “A Paranigral VTA Nociceptin Circuit that Constrains Motivation for Reward”. Cell Jul, 2019. doi: 10.1016/j.cell.2019.06.034

Choi … McNally, et al “Paraventricular Thalamus Controls Behavior during Motivational Conflict”. The Journal of Neuroscience Jun, 2019. doi: 10.1523/jneurosci.2480-18.2019

Feng … Li, et al “A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.02.037

 

Cameron … Witten, et al “Increased Cocaine Motivation Is Associated with Degraded Spatial and Temporal Representations in IL-NAc Neurons”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.04.015

Bai … Knight, et al “Genetic Identification of Vagal Sensory Neurons That Control Feeding.”. Cell , 2019. doi: 10.1016/j.cell.2019.10.031

Robinson … Gradinaru, et al “Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1”. eLife , 2019. doi: 10.7554/elife.48983

Pascoli … Lüscher, et al “Stochastic synaptic plasticity underlying compulsion in a model of addiction”. Nature Dec, 2018. doi: 10.1038/s41586-018-0789-4

Reed … Britt, et al “Coordinated Reductions in Excitatory Input to the Nucleus Accumbens Underlie Food Consumption”. Neuron Sep, 2018. doi: 10.1016/j.neuron.2018.07.051

Walsh … Malenka, et al “5-HT release in nucleus accumbens rescues social deficits in mouse autism model”. Nature Aug, 2018. doi: 10.1038/s41586-018-0416-4

Saunders … Janak, et al “Dopamine neurons create Pavlovian conditioned stimuli with circuit-defined motivational properties”. Nature Neuroscience Jul, 2018. doi: 10.1038/s41593-018-0191-4

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Flexible Configurations for the RZ10X

Record from Multiple Subjects or Sites

The RZ10X can record from two subjects or sites simultaneously. Easily customize and modify your RZ10X for your changing experiments.

 

Record from Multiple Fluorophores

Record GCaMP and RCaMP responses from up to two sites with interchangeable LEDs. Also stimulate simultaneously with optogenetics.

Record from Multiple Subjects | TDT's Fiber Photometry System

 

Record from Multiple Sites | TDT's Fiber Photometry System

 

 

 

 

 

 

 

Detect, Connect and Go!

The Fiber Photometry Gizmo streamlines running experiments in Synapse for the RZ10X.

Real-Time Signal Visualization

Removing motion artifact is easy with our real-time signal processor. A few clicks and the ΔF/F can be calculated to confirm a positive response from your animals during experimental paradigms. Another click and the isosbestic signal (with motion artifact) can be removed from the GCaMP response for a normalized ΔF/F.

Additional functionalities include an integrated power meter and fiber bleaching.

Fiber Photometry Gizmo

LUX Behavioral Integration

Behavior

Integrate third party behavioral codes from multiple operant boxes with your fiber photometry recordings and view evoked responses in real-time.

LUX Video Integration

Video

Synchronize video recordings from up to two USB cameras. Incorporate scoring and timestamps both online and offline.

LUX EEG Integration

Electrophysiology

Record neural responses, including single and multi-unit activity, with TDT’s PZ5 Amplifier or Medusa Amplifier  (up to 32 channels).

Data Import and Analysis

Offline Analysis

 Synapse data can be exported to Matlab, Python, Excel, or another analysis software of your choice. With TDT, you no longer need to worry about precisely aligning behavioral codes with neural responses. The integration of your behavioral, video, and fiber photometry data into one data structure ensures consistent timing and minimal synchronization errors.

Check out the Specs & Resources tab or Matlab Workbooks for offline analysis code and examples.

Real-time Lock-In Amplification

Lock-in amplification is a signal processing technique that improves the signal-to-noise ratio of fiber photometry. With lock-in amplification, small fluorophore responses can be detected and extracted from the raw signal in real-time.

How Does Lock-In Amplification Work?

LEDs are driven at a fixed, sinusoidal frequency (E1 and E2) through a fiber optic cable. The brain target is then excited to record the fluorophore responses (F1 and F2). The raw signal is detected, but the fluorophore responses are obscured by the background noise.

Lock-in amplification is then applied and uses the carrier frequency of the LEDs to extract the fluorophore signal from the noise of the raw signal. In addition, multiple LEDs can be driven at different frequencies. This allows for the simultaneous detection of multiple signals, including GCaMP and isosbestic.

Webinar

Introduction to the RZ10X for Fiber Photometry

Learn more about TDT’s integrated fiber photometry, and how to leverage this system for GCaMP and dLight recordings.

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Commitment to Your Research

We understand that every experiment is dependent on your specific research. TDT service professionals work with one job in mind: to ensure that you get the help you need.

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