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Fiber Photometry System

Conduct calcium imaging experiments with our fiber photometry processor and Synapse software.

Description

Specs & Resources

Publications

Fiber Photometry experiments have never been so easy to set up and integrate into other research projects.

The TDT Fiber Photometry System features the RZ10 acquisition processor and TDT’s Synapse software. Our Fiber Photometry System is designed specifically for researchers wanting to perform quality calcium imaging and optogenetics experiments while having access to electrophysiology and control of external behavioral devices.

Synapse and our real-time hardware architecture allow for seamless integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with minimal latency. Correlate neural responses to behavioral states or to external stimuli, such as sound, sight, and smell.

  • Zimmer, et al “Functional Ontogeny of Hypothalamic Agrp Neurons in Neonatal Mouse Behaviors.” Cell May 16, 2019. doi: 10.1016/j.cell.2019.04.026.
  • Holly, et al “Striatal Low-Threshold Spiking Interneurons Regulate Goal-Directed Learning.” Neuron 2019, May 13. doi: 10.1016/j.neuron.2019.04.016.
  • Choi, et al “Paraventricular thalamus controls behavior during motivational conflict.” Journal of Neuroscience 12 April 2019, 2480-18. doi: 10.1523/JNEUROSCI.2480-18.2019.
  • He, et al “Cellular and synaptic reorganization of arcuate NPY/AgRP and POMC neurons after exercise.” Molecular Metabolism (18):Dec 2018:107-119. doi: 10.1016/j.molmet.2018.08.011.
  • Sengupta, et al “Basolateral Amygdala Neurons Maintain Aversive Emotional Salience.” Journal of Neuroscience March 2018, 38 (12) 3001-3012. doi: 10.1523/JNEUROSCI.2460-17.2017.

Additional fiber photometry publications

 

Record from Multiple Subjects | TDT's Fiber Photometry System

 

 

Record from Multiple Subjects

The flexibility of our fiber photometry system means that multiple fiber optics can be sent to multiple subjects. Have independent GCaMP + isosbestic control of up to three animals, or independent GCaMP control up to four animals.

Record from Multiple Sites | TDT's Fiber Photometry System

 

Record from Multiple Sites

Send multiple single or branching fiber patch cords to different sites for dissecting the dynamics of neural circuitry on a wider scale. You have independent control of three (RZ10) to six (RZ10x) LED sources with the RZ10(x) DAC outputs.

 

Record Other Neurophysiological Signals | TDT's Fiber Photometry System


Record Other Neurophysiological Signals

The RZ10 + Synapse is a flexible platform that allows for easy integration of electrophysiology and other signals into your current experiments. The RZ10(x) comes with pre-built optical ports to add a Medusa or PZ5 amplifier (RZ10x only) for doing 4 – 32 channels of ephys. Other analog signals can be captured by the remaining ADC inputs of the RZ10.

Lock-In Amplification

See many clear demodulated fluorophore responses in real-time.

Our fiber photometry system works off the principle of locked-in amplification, wherein LED light sources are driven at high frequencies (200 – 500 Hz) and low-frequency fluorescent responses are demodulated out of the raw photostream.

Technique

Lock-in amplification is a signal processing technique that uses modulation of driver signals and an orthogonal reference signal (cosine of the driver signal) to extract relevant amplitude and phase of frequency-specific responses in a complex and often noisy signal. We drive multiple signals at different frequencies and then extract only the contribution from each of those frequencies in the acquired photodetector signal – that’s how we can detect multiple fluorescent simultaneously off of a single photosource.

Benefits

Lock-in amplification has many benefits. First, it allows for high-fidelity signal detection in noisy environments. Since signals are extracted based on their frequency content, the fluorescent signal is not affected by parasitic room lighting or electronic noise. Additionally, this technique allows for detecting several signals simultaneously off the same fiber optic cable (405 isosbestic, 465 GCaMP, 560 tdTomato, etc).

Difference

Other systems use CMOS cameras or light spectroscopy to pick up fluorescent signal responses. This normally requires multiple fibers per light source and is a generally noisier technique. Our lock-in amplification makes it easy to send multiple light sources down the same fiber to detect several independent signals from the same site. No need for multiple fibers – one site, several colors, a clean signal!

Fiber Photometry Experiment Gizmos

TDT Engineers brought the RZ10 Lux Integrated Fiber Photometry Processor from concept to production and worked with our technical support team to ensure the Fiber Photometry Gizmo is relatively easy to use for any lab.

Anyone can master Fiber Photometry setup with relative ease. Connect and control all of your other S3 devices with ease using the intuitive Synapse drop-and-go gizmo functionality.

Fiber Photometry Gizmo

Data Import and Analysis

Extract the photometry signal and analyze the fluorescence output.

With TDT’s new MatlabSDK or PythonSDK, your data will be imported as a structure for straightforward handling of all streams, snips, and epoc events. There are many optional functions in the SDK, such as TDTfilter, which lets you filter data around epoc events to make peri-event response plots. You can also look at our Offline Analysis Matlab Workbooks, which are full-code examples of how to import data into Matlab and do interesting things.

There are also optional exports to ascii or other formats for analysis in Excel.

Commitment to Your Research

We understand that every experiment is dependent on your specific research. TDT service professionals work with one job in mind: to ensure that you get the help you need.

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