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Fiber Photometry System

Conduct calcium imaging experiments with our fiber photometry processor and Synapse software.

Description

Specs & Resources

Publications

The TDT Fiber Photometry System features the RZ5P acquisition processor and our flagship Synapse software. Designed specifically to fit the growing needs of customers who wanted to perform quality calcium imaging and optogenetics experiments with access to electrophysiology and control of external behavioral devices.

Our software and hardware allow for seamless integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with no delay from our real-time processor. Correlate neural responses to behavioral states as well as external stimuli such as sound, sight and smell.

Fiber Photometry Experiments have never been so easy to setup and simultaneously run with other research projects.

Fiber Photometry User Guide

  • Zimmer, et al “Functional Ontogeny of Hypothalamic Agrp Neurons in Neonatal Mouse Behaviors.” Cell May 16, 2019. doi: 10.1016/j.cell.2019.04.026.
  • Holly, et al “Striatal Low-Threshold Spiking Interneurons Regulate Goal-Directed Learning.” Neuron 2019, May 13. doi: 10.1016/j.neuron.2019.04.016.
  • Choi, et al “Paraventricular thalamus controls behavior during motivational conflict.” Journal of Neuroscience 12 April 2019, 2480-18. doi: 10.1523/JNEUROSCI.2480-18.2019.
  • He, et al “Cellular and synaptic reorganization of arcuate NPY/AgRP and POMC neurons after exercise.” Molecular Metabolism (18):Dec 2018:107-119. doi: 10.1016/j.molmet.2018.08.011.
  • Sengupta, et al “Basolateral Amygdala Neurons Maintain Aversive Emotional Salience.” Journal of Neuroscience March 2018, 38 (12) 3001-3012. doi: 10.1523/JNEUROSCI.2460-17.2017.

Additional fiber photometry publications

System Components

RZ5P: Fiber Photometry Processor

Synapse Suite

PO5e Interface Card

Connect & Turn On

Connect Sensors & Drivers and turn on RZ5P Fiber Photometry Processor with Synapse Software.

TDT Fiber Photometry (RZ5P) processor controls the voltage signal for the LED light driver, captures the photoreceiver response, and performs online real-time demodulation.
Connect the LED driver and light sources to the RZ5P outputs and the photoreceiver to the RZ5P input with BNC cables, and fiber-optic patch cords between all the light I/O modules.

Drop & Go Synapse Gizmo

To make your life easier, Fiber Photometry has been encapsulated in a single gizmo, found under the neural group in the gizmo list.

Synapse software controls the signal parameters on the RZ5P and reads the data for viewing and storage.
The RZ5 platform features two or three ultra fast digital signal processors for optimal performance. Since Synapse will be handling your digital signal processor (DSP) details, like assigning tasks to the DSP and distributing tasks evenly, you only need to worry about higher level functions like organizing multiple experiments across numerous subjects and various lab members

Press Record

Just like that, begin collecting and visualizing real-time data on fiber photometric responses.

Now, easily integrate video recording, behavioral study, electrophysiology recordings, and optogenetic stimulation. Any combination of these modalities can be run simultaneously, and with these results, you can further bolster your hypotheses for your research.

 

 

 

Record from Multiple Subjects

The flexibility of our fiber photometry system means that multiple fiber optics can be sent to multiple subjects. Have independent GCaMP + isosbestic control of up to two animals, or independent GCaMP control up to four animals.

 

Record from Multiple Sites

Send multiple single or branching fiber patch cords to different sites for dissecting the dynamics of neural circuitry on a wider scale. You have independent control of up to four LED sources with the RZ5P DAC outputs.

 


Record Other Neurophysiological Signals

The RZ5P + Synapse is a flexible platform that allows for easy integration of electrophysiology and other signals to your current experiment and recordings. The RZ5P comes pre-built with the capability to add on a PZ5 amplifier for doing up to 32 channels of ephys. Other analog signals can be captured by the remaining ADC inputs of the RZ5P.

Lock-In Amplification

See many clear demodulated fluorophore responses in real time.

Our fiber photometry system works off the principle of locked-in amplification, wherein LED lightsources are driven at high frequencies (200 – 500 Hz) and low-frequency fluorescent responses are demodulated out of the raw photostream.

Technique

Lock-in amplification is a signal processing technique that uses modulation of driver signals and an orthogonal reference signal (cosine of the driver signal) to extract relevant amplitude and phase of frequency-specific responses in a complex and often noisy signal. We drive multiple signals at different frequencies and then extract only the contribution from each of those frequencies in the acquired photodetector signal – that’s how we can detect multiple fluorescent simultaneously off of a single photosource.

Benefits

Lock-in amplification has many benefits. First, it allows for high-fidelity signal detection in noisy environments. Since signals are extracted based on their frequency content, the fluorescent signal is not affected by parasitic room lighting or electronic noise. Additionally, this technique allows for detecting several signals simultaneously off the same fiber optic cable (405 isobestic, 465 GCaMP, 560 TDtomato, etc).

Difference

Other systems use CMOS cameras or light spectroscopy to pick up fluorescent signal responses. This normally requires multiple fibers per light source and is a generally noisier technique. Our lock-in amplification makes it easy to send multiple light sources down the same fiber to detect several independent signals from the same site. No need for multiple fibers – one site, several colors, clean signal!

Fiber Photometry Experiment Gizmos

TDT Engineers brought the RZ5P Fiber Photometry Processor from concept to production and worked with our technical support team to ensure the Fiber Photometry Gizmo is relatively easy to use for any lab.

Even the novice at Fiber Photometry experiments can master the technique relatively easily. With the Drop and Go Intuitive Gizmo functionality, the Synapse software allows you to easily connect other devices and directly control all of your S3 Devices.

Fiber Photometry Gizmo

Data Import and Analysis

Extract the photometry signal and analyze the fluorescence output.

With TDT’s new MatlabSDK, your data will be imported as a structure for straightforward handling of all streams, snips, and epoc events. There are many optional functions in the SDK, such as TDTfilter, which lets you filter data around epoc events to make peri-event response plots. You can also look at our Offline Analysis Matlab Workbooks, which are full-code examples of how to import data into Matlab and do interesting things.

There are also optional exports to ascii or other formats for analysis in Excel.

Commitment to Your Research

We understand that every experiment is dependent on your specific research. TDT service professionals work with one job in mind: to ensure that you get the help you need.

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