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Fiber Photometry System

Perform Three-Color Photometry with the iX7

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Specs & Resources

Publications

Fiber Photometry is a recording technique that uses low-powered light from LEDs to detect calcium or neurotransmitter activity in cell populations via genetically encoded fluorescent indicators (GEFIs) [10.1016/j.neuron.2023.11.016]. Neurons, glial cells, and astrocytes can be targeted with viral vectors so that only specific receptor types express fluorescent proteins. This localization and easy chronic recording setup makes fiber photometry a great choice for studying the roles of select brain nuclei and neurotransmitters in behavioral assays.

TDT is the long-time industry leader in fiber photometry. 

Our turn-key optical solutions have been used by researchers to pioneer the field of fiber photometry. Our lock-in amplifier systems are proven to be the most sensitive and versatile systems on the market.

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2022     * RZ10X

* Goldstein, … Betley, et al. (2022). “Specificity of Varenicline in Blocking Mesolimbic Circuit Activation to Natural and Drug Rewards”. Neuroscience, 483. https://doi.org/10.1016/J.NEUROSCIENCE.2021.12.016

Liu, … Anderson, et al. (2022). “Make war not love: The neural substrate underlying a state-dependent switch in female social behavior”. Neuron. https://doi.org/10.1016/j.neuron.2021.12.002

Wu, … Halpern, et al. (2022). “Local accumbens in vivo imaging during deep brain stimulation reveals a strategy-dependent amelioration of hedonic feeding”. Proceedings of the National Academy of Sciences, 119(1). https://doi.org/10.1073/pnas.2109269118

 

2021     * RZ10X

* Copits, … Bruchas, et al. (2021). “A photoswitchable GPCR-based opsin for presynaptic inhibition”. Neuron, 109(11). https://doi.org/10.1016/J.NEURON.2021.04.026

* Ferrara, … Rosenkranz, et al. (2021). “Developmental Shifts in Amygdala Activity during a High Social Drive State”. Journal of Neuroscience, 41(45). https://doi.org/10.1523/JNEUROSCI.1414-21.2021

* Horio, Liberles. (2021) “Hunger enhances food-odour attraction through a neuropeptide Y spotlight”. Nature, 592(7853). https://doi.org/10.1038/s41586-021-03299-4

Samineni, … Gereau, et al. (2021). “Cellular, circuit and transcriptional framework for modulation of itch in the central amygdala”. ELife, 10. https://doi.org/10.7554/eLife.68130

Robert, … Polley, et al. (2021). “A functional topography within the cholinergic basal forebrain for encoding sensory cues and behavioral reinforcement outcomes”. ELife, 10. https://doi.org/10.7554/eLife.69514

Reichenbach, … Andrews, et al. (2021). “Glucose-sensing in AgRP neurons integrates homeostatic energy state with dopamine signalling in the striatum”. BioRxiv. https://doi.org/10.1101/2021.03.22.436393

Tian, … Beier, et al. (2021). “An extended amygdala-midbrain circuit controlling cocaine withdrawal-induced anxiety and reinstatement”. BioRxiv. https://doi.org/10.1101/2021.11.05.467532

Chiacchierini, … McCutcheon, et al. (2021). “Protein Appetite Drives Macronutrient-Related Differences in Ventral Tegmental Area Neural Activity”. The Journal of Neuroscience, 41(23). https://doi.org/10.1523/JNEUROSCI.3082-20.2021

Choi, … McNally, et al. (2021). “A corticothalamic circuit trades off speed for safety during decision-making under motivational conflict”. BioRxiv. https://doi.org/10.1101/2021.11.21.469477

Feng, … He, et al. (2021). “The entorhinal cortex modulates trace fear memory formation and neuroplasticity in the lateral amygdala via cholecystokinin”. BioRxiv. https://doi.org/10.1101/2021.04.18.440346

Penzo, … Chudasama, et al. (2021). “Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of reactive and proactive defensive behaviors”. Research Square. https://doi.org/10.21203/rs.3.rs-322756/v1

Ghareh, … Marchant, et al. (2021). “Role of anterior insula cortex in context-induced relapse of nicotine-seeking”. BioRxiv. https://doi.org/10.1101/2021.12.08.471717

Holly, … Fuccillo, et al. (2021). “Striatal low-threshold spiking interneurons locally gate dopamine”. Current Biology, 31(18). https://doi.org/10.1016/J.CUB.2021.06.081

Wang, … Pitt, et al. (2021). “Scn2a severe hypomorphic mutation decreases excitatory synaptic input and causes autism-associated behaviors”. JCI Insight, 6(15). https://doi.org/10.1172/jci.insight.150698

Luchsinger, … Centanni, et al. (2021). “Delineation of an insula-BNST circuit engaged by struggling behavior that regulates avoidance in mice”. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-23674-z

van Zessen, … Lüscher, et al. (2021). “Dynamic dichotomy of accumbal population activity underlies cocaine sensitization”. ELife, 10. https://doi.org/10.7554/eLife.66048

McGovern, … Root, et al. (2021). “Neurochemical Signaling of Reward and Aversion to Ventral Tegmental Area Glutamate Neurons”. The Journal of Neuroscience, 41(25). https://doi.org/10.1523/JNEUROSCI.1419-20.2021

Yau, … McNally, et al. (2021). “The Roles of Basolateral Amygdala Parvalbumin Neurons in Fear Learning”. The Journal of Neuroscience, 41(44). https://doi.org/10.1523/JNEUROSCI.2461-20.2021

Melchior, … Winder, et al. (2021). “Cocaine Augments Dopamine-Mediated Inhibition of Neuronal Activity in the Dorsal Bed Nucleus of the Stria Terminalis”. Journal of Neuroscience, 41(27). https://doi.org/10.1523/JNEUROSCI.0284-21.2021

Smiley, … Gass, et al. (2021). “Adolescent exposure to delta-9-tetrahydrocannabinol and ethanol heightens sensitivity to fear stimuli”. Behavioural Brain Research, 415. https://doi.org/10.1016/J.BBR.2021.113517

Zhang, … Ma, et al. (2021). “Ventral striatal islands of Calleja neurons control grooming in mice”. Nature Neuroscience, 24(12). https://doi.org/10.1038/s41593-021-00952-z

Thirtamara Rajamani, … Harony-Nicolas, et al. (2021). “Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation”. Scientific Reports 2021 11:1, 11(1). https://doi.org/10.1038/s41598-021-01818-x

Martyniuk, … Kellendonk, et al. (2021). “Dopamine D2Rs Coordinate Cue-Evoked Changes in Striatal Acetylcholine Levels”. BioRxiv. https://doi.org/10.1101/2021.12.08.471871

He, … Xu, et al. (2021). “5-HT recruits distinct neurocircuits to inhibit hunger-driven and non-hunger-driven feeding”. Molecular Psychiatry. https://doi.org/10.1038/s41380-021-01220-z

Jung, … Kim, et al. (2021). “A forebrain neural substrate for behavioral thermoregulation”. Neuron. https://doi.org/10.1016/j.neuron.2021.09.039

Jean-Richard-dit-Bressel, … McNally, et al. (2021). “Instrumental aversion coding in the basolateral amygdala and its reversion by a benzodiazepine”. Neuropsychopharmacology 2021. https://doi.org/10.1038/s41386-021-01176-2

Froemke, … Jung, et al. (2021). “Neural circuitry for maternal oxytocin release induced by infant cries”. Research Square. https://doi.org/10.21203/rs.3.rs-970204/v1

Kutlu, … Calipari, et al. (2021). “Dopamine release in the nucleus accumbens core signals perceived saliency”. Current Biology, 31(21). https://doi.org/10.1016/j.cub.2021.08.052

Goldstein, … Alhadeff, et al. (2021). “Hypothalamic detection of macronutrients via multiple gut-brain pathways”. Cell Metabolism, 33(3). https://doi.org/10.1016/j.cmet.2020.12.018

Spring, … Wheeler, et al. (2021). “Chronic Stress Prevents Cortico-Accumbens Cue Encoding and Alters Conditioned Approach”. The Journal of Neuroscience, 41(11). https://doi.org/10.1523/JNEUROSCI.1869-20.2021

Dong, … Williams, et al. (2021). “Time and metabolic state-dependent effects of GLP-1R agonists on NPY/AgRP and POMC neuronal activity in vivo”. Molecular Metabolism, 54. https://doi.org/10.1016/j.molmet.2021.101352

Park, … Kim, et al. (2021). “Social isolation impairs the prefrontal-nucleus accumbens circuit subserving social recognition in mice”. Cell Reports, 35(6). https://doi.org/10.1016/j.celrep.2021.109104

He, … Xu, et al. (2021). “Barbadin Potentiates Long-Term Effects of Lorcaserin on POMC Neurons and Weight Loss”. The Journal of Neuroscience, 41(26). https://doi.org/10.1523/JNEUROSCI.3210-20.2021

Norman, … Morishita, et al. (2021). “Post-error recruitment of frontal sensory cortical projections promotes attention in mice”. Neuron, 109. https://doi.org/10.1016/j.neuron.2021.02.001

Sherathiya, … Lerner, et al. (2021). “GuPPy, a Python toolbox for the analysis of fiber photometry data”. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-03626-9

de Kloet … Mansvelder, et al “Bi-directional regulation of cognitive control by distinct prefrontal cortical output neurons to thalamus and striatum”. Nature Communications Mar, 2021. doi: 10.1038/s41467-021-22260-7

Stern … Friedman, et al “Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons”. Cell Metabolism Mar, 2021. doi: 10.1016/j.cmet.2021.03.001

Peters … McCutcheon, et al “Distracting stimuli evoke ventral tegmental area responses in rats during ongoing saccharin consumption”. European Journal of Neuroscience Mar, 2021. doi: 10.1111/ejn.15108

Sathyanesan … Gallo, et al “Disruption of neonatal Purkinje cell function underlies injury-related learning deficits”. Proceedings of the National Academy of Sciences Mar, 2021. doi: 10.1073/pnas.2017876118

Bruno … Barker, et al “pMAT: An open-source software suite for the analysis of fiber photometry data”. Pharmacology Biochemistry and Behavior Feb, 2021. doi: 10.1016/j.pbb.2020.173093

Mayer … Blakely, et al “There’s no place like home? Return to the home cage triggers dopamine release in the mouse nucleus accumbens”. Neurochemistry International Jan, 2021. doi: 10.1016/j.neuint.2020.104894

Karigo … Anderson, et al “Distinct hypothalamic control of same- and opposite-sex mounting behaviour in mice”. Nature Jan, 2021. doi: 10.1038/s41586-020-2995-0

 

2020

Seiler, … Lerner, et al. (2020). “Dopamine signaling in the dorsomedial striatum promotes compulsive behavior”. BioRxiv. https://doi.org/10.1101/2020.03.30.016238

Alabi, … Fuccillo, et al. (2020). “Disruption of Nrxn1α within excitatory forebrain circuits drives value-based dysfunction”. ELife, 9. https://doi.org/10.7554/eLife.54838

Sofia Beas … Penzo, et al “A ventrolateral medulla-midline thalamic circuit for hypoglycemic feeding”. Nature Communications Dec, 2020. doi: 10.1038/s41467-020-19980-7

He … Xu, et al “Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance”. Nature Communications Dec, 2020. doi: 10.1038/s41467-020-15982-7

Hsu … Roitman, et al “Thirst recruits phasic dopamine signaling through subfornical organ neurons”. Proceedings of the National Academy of Sciences Dec, 2020. doi: 10.1073/pnas.2009233117

Bicks … Morishita, et al “Prefrontal parvalbumin interneurons require juvenile social experience to establish adult social behavior”. Nature Communications Dec, 2020. doi: 10.1038/s41467-020-14740-z

Jaramillo … Centanni, et al “BNST transient activity associates with approach behavior in a stressful environment and is modulated by the parabrachial nucleus”. Neurobiology of Stress Nov, 2020. doi: 10.1016/j.ynstr.2020.100247

Miletta … Horvath, et al “AgRP neurons control compulsive exercise and survival in an activity-based anorexia model”. Nature Metabolism Nov, 2020. doi: 10.1038/s42255-020-00300-8

Yamamuro … Morishita, et al “A prefrontal–paraventricular thalamus circuit requires juvenile social experience to regulate adult sociability in mice”. Nature Neuroscience Oct, 2020. doi: 10.1038/s41593-020-0695-6

Root … Morales, et al “Distinct Signaling by Ventral Tegmental Area Glutamate, GABA, and Combinatorial Glutamate-GABA Neurons in Motivated Behavior”. Cell Reports Sep, 2020. doi: 10.1016/j.celrep.2020.108094

Liu … McNally, et al “The Mesolimbic Dopamine Activity Signatures of Relapse to Alcohol-Seeking”. The Journal of Neuroscience Aug, 2020. doi: 10.1523/jneurosci.0724-20.2020

Barbano … Morales, et al “VTA Glutamatergic Neurons Mediate Innate Defensive Behaviors”. Neuron Jul, 2020. doi: 10.1016/j.neuron.2020.04.024

Cho … Sohal, et al “Cross-hemispheric gamma synchrony between prefrontal parvalbumin interneurons supports behavioral adaptation during rule shift learning”. Nature Neuroscience Jul, 2020. doi: 10.1038/s41593-020-0647-1

Gadziola … Wesson, et al “A Neural System that Represents the Association of Odors with Rewarded Outcomes and Promotes Behavioral Engagement”. Cell Reports Jul, 2020. doi: 10.1016/j.celrep.2020.107919

Jacobs, Moghaddam “Prefrontal Cortex Representation of Learning of Punishment Probability During Reward-Motivated Actions”. The Journal of Neuroscience Jun, 2020. doi: 10.1523/jneurosci.0310-20.2020

Steinberg … Malenka, et al “Amygdala-Midbrain Connections Modulate Appetitive and Aversive Learning”. Neuron Jun, 2020. doi: 10.1016/j.neuron.2020.03.016

Salimando … Winder, et al “BNST GluN2D-Containing NMDA Receptors Influence Anxiety- and Depressive-like Behaviors and ModulateCell-Specific Excitatory/Inhibitory Synaptic Balance”. The Journal of Neuroscience May, 2020. doi: 10.1523/jneurosci.0270-20.2020

Tan … Zuker, et al “The gut–brain axis mediates sugar preference”. Nature Apr, 2020. doi: 10.1038/s41586-020-2199-7

Berland … Luquet, et al “Circulating Triglycerides Gate Dopamine-Associated Behaviors through DRD2-Expressing Neurons”. Cell Metabolism Apr, 2020. doi: 10.1016/j.cmet.2020.02.010

Lafferty … Britt, et al “Nucleus Accumbens Cell Type- and Input-Specific Suppression of Unproductive Reward Seeking”. Cell Reports Mar, 2020. doi: 10.1016/j.celrep.2020.02.095

Pignatelli … Bonci, et al “Cooperative synaptic and intrinsic plasticity in a disynaptic limbic circuit drive stress-induced anhedonia and passive coping in mice”. Molecular Psychiatry Mar, 2020. doi: 10.1038/s41380-020-0686-8

Corkrum … Araque, et al “Dopamine-Evoked Synaptic Regulation in the Nucleus Accumbens Requires Astrocyte Activity”. Neuron Mar, 2020. doi: 10.1016/j.neuron.2019.12.026

Konanur … Roitman, et al “Phasic dopamine responses to a food-predictive cue are suppressed by the glucagon-like peptide-1 receptor agonist Exendin-4”. Physiology & Behavior Mar, 2020. doi: 10.1016/j.physbeh.2019.112771

Marcus … Patel, et al “Endocannabinoid Signaling Collapse Mediates Stress-Induced Amygdalo-Cortical Strengthening”. Neuron Mar, 2020. doi: 10.1016/j.neuron.2019.12.024

Gao … Penzo, et al “Two genetically, anatomically and functionally distinct cell types segregate across anteroposterior axis of paraventricular thalamus”. Nature Neuroscience Feb, 2020. doi: 10.1038/s41593-019-0572-3

Salimando … Winder, et al “BNST GluN2D-Containing NMDA Receptors Influence Anxiety-and Depressive-like Behaviors and Modulate Cell-Specific Excitatory/Inhibitory Synaptic Balance”. The Journal of Neuroscience , 2020. doi: 10.1523/jneurosci.0270-20.2020

 

2019

Heifets … Malenka, et al “Distinct neural mechanisms for the prosocial and rewarding properties of MDMA”. Science Translational Medicine Dec, 2019. doi: 10.1126/scitranslmed.aaw6435

Mendoza … Britt, et al “Cue-Evoked Dopamine Neuron Activity Helps Maintain but Does Not Encode Expected Value”. Cell Reports Nov, 2019. doi: 10.1016/j.celrep.2019.09.077

Alhadeff … Betley, et al “Natural and Drug Rewards Engage Distinct Pathways that Converge on Coordinated Hypothalamic and Reward Circuits”. Neuron Sep, 2019. doi: 10.1016/j.neuron.2019.05.050

Shi … Fu, et al “A Rare Mutation of β1-Adrenergic Receptor Affects Sleep/Wake Behaviors”. Neuron Sep, 2019. doi: 10.1016/j.neuron.2019.07.026

Sengupta, Holmes “A Discrete Dorsal Raphe to Basal Amygdala 5-HT Circuit Calibrates Aversive Memory”. Neuron Aug, 2019. doi: 10.1016/j.neuron.2019.05.029

Guo … Polley, et al “The Cholinergic Basal Forebrain Links Auditory Stimuli with Delayed Reinforcement to Support Learning”. Neuron Jul, 2019. doi: 10.1016/j.neuron.2019.06.024

Parker … Bruchas, et al “A Paranigral VTA Nociceptin Circuit that Constrains Motivation for Reward”. Cell Jul, 2019. doi: 10.1016/j.cell.2019.06.034

Choi … McNally, et al “Paraventricular Thalamus Controls Behavior during Motivational Conflict”. The Journal of Neuroscience Jun, 2019. doi: 10.1523/jneurosci.2480-18.2019

Feng … Li, et al “A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.02.037

 

Cameron … Witten, et al “Increased Cocaine Motivation Is Associated with Degraded Spatial and Temporal Representations in IL-NAc Neurons”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.04.015

Mansy … Oweiss, et al “Spatial detection characteristics of a single photon fiber photometry system for imaging neural ensembles *”. 2019 9th International IEEE/EMBS Conference on Neural Engineering (NER) Mar, 2019. doi: 10.1109/ner.2019.8717005

Zimmer … Dietrich, et al “Functional Ontogeny of Hypothalamic Agrp Neurons in Neonatal Mouse Behaviors”. Cell , 2019. doi: 10.1016/j.cell.2019.04.026

Holly … Fuccillo, et al “Striatal Low-Threshold Spiking Interneurons Regulate Goal-Directed Learning”. Neuron , 2019. doi: 10.1016/j.neuron.2019.04.016

Li … Krashes, et al “Defined Paraventricular Hypothalamic Populations Exhibit Differential Responses to Food Contingent on Caloric State”. Cell Metabolism , 2019. doi: 10.1016/j.cmet.2018.10.016

Robinson … Gradinaru, et al “Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1”. eLife , 2019. doi: 10.7554/elife.48983

Bai … Knight, et al “Genetic Identification of Vagal Sensory Neurons That Control Feeding.”. Cell , 2019. doi: 10.1016/j.cell.2019.10.031

 

2018

Pascoli … Lüscher, et al “Stochastic synaptic plasticity underlying compulsion in a model of addiction”. Nature Dec, 2018. doi: 10.1038/s41586-018-0789-4

Reed … Britt, et al “Coordinated Reductions in Excitatory Input to the Nucleus Accumbens Underlie Food Consumption”. Neuron Sep, 2018. doi: 10.1016/j.neuron.2018.07.051

Walsh … Malenka, et al “5-HT release in nucleus accumbens rescues social deficits in mouse autism model”. Nature Aug, 2018. doi: 10.1038/s41586-018-0416-4

Saunders … Janak, et al “Dopamine neurons create Pavlovian conditioned stimuli with circuit-defined motivational properties”. Nature Neuroscience Jul, 2018. doi: 10.1038/s41593-018-0191-4

Giza … Lee, et al “The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior”. Neuron Jul, 2018. doi: 10.1016/j.neuron.2018.05.024

Beas … Penzo, et al “The locus coeruleus drives disinhibition in the midline thalamus via a dopaminergic mechanism”. Nature Neuroscience Jun, 2018. doi: 10.1038/s41593-018-0167-4

Fang … Lin, et al “A Hypothalamic Midbrain Pathway Essential for Driving Maternal Behaviors”. Neuron Apr, 2018. doi: 10.1016/j.neuron.2018.02.019

Sengupta … McNally, et al “Basolateral Amygdala Neurons Maintain Aversive Emotional Salience”. The Journal of Neuroscience Mar, 2018. doi: 10.1523/jneurosci.2460-17.2017

Augustine … Oka, et al “Hierarchical neural architecture underlying thirst regulation”. Nature Mar, 2018. doi: 10.1038/nature25488

Wang … Krauzlis, et al “Activation of Striatal Neurons Causes a Perceptual Decision Bias during Visual Change Detection in Mice”. Neuron Mar, 2018. doi: 10.1016/j.neuron.2018.01.049

Chen … Dan, et al “A Hypothalamic Switch for REM and Non-REM Sleep”. Neuron Mar, 2018. doi: 10.1016/j.neuron.2018.02.005

 

2017

Hashikawa … Lin, et al “Esr1+ cells in the ventromedial hypothalamus control female aggression”. Nature Neuroscience Nov, 2017. doi: 10.1038/nn.4644

Barker … Morales, et al “Lateral Preoptic Control of the Lateral Habenula through Convergent Glutamate and GABA Transmission”. Cell Reports Nov, 2017. doi: 10.1016/j.celrep.2017.10.066

Calipari … Nestler, et al “Dopaminergic dynamics underlying sex-specific cocaine reward”. Nature Communications Apr, 2017. doi: 10.1038/ncomms13877

 

2016

Wells … Halassa, et al “Thalamic reticular impairment underlies attention deficit in Ptchd1Y/− mice”. Nature Mar, 2016. doi: 10.1038/nature17427

Zalocusky … Deisseroth, et al “Nucleus accumbens D2R cells signal prior outcomes and control risky decision-making”. Nature Mar, 2016. doi: 10.1038/nature17400

Calipari … Nestler, et al “In vivo imaging identifies temporal signature of D1 and D2 medium spiny neurons in cocaine reward”. Proceedings of the National Academy of Sciences of the United States of America , 2016. doi: 10.1073/pnas.1521238113

 

2015

Wimmer … Halassa, et al “Thalamic control of sensory selection in divided attention”. Nature Oct, 2015. doi: 10.1038/nature15398

Fiber photometry hardware has never been this straightforward

Simple Subject Connection

Easily connect your subject patch cord to the iX7 directly or with a commercial rotary joint for freely-moving behavior.

Record RED and GREEN fluorescent responses simultaneously in just a few clicks of our Synapse software. Everything auto-detects so you can start recording right away.

Our built-in Q-factor fluorescence detection and power meter make checking signal quality easier than all other existing systems.

 

Real-time Lock-In Amplification

TDT Fiber Photometry systems use a technique called ‘Lock-in Amplification’ to detect very small changes in fluorescence from active neurons.

How Does Lock-In Amplification Work?

This works by modulating different wavelength LEDs (e.g. 415 nm, 465 nm, 560 nm) at different sinusoidal frequencies and sending them through a series of optical filters and dichroic mirrors. The lights are on simultaneously and do not interfere with one another, unlike CMOS-based imaging systems.

Lock-in amplification is then applied and uses the carrier frequency of the LEDs to extract the fluorophore signal from the noise of the raw signal. In addition, multiple LEDs can be driven at different frequencies. This allows for the simultaneous detection of multiple signals like GFP, RFP, and isosbestic controls.

Detect, Connect and Go!

Real-time demodulation and dFF calculations, closed-loop optogenetic stimulation – Synapse can do it all. Every aspect of the iX7 is configurable with just a few mouse clicks. Go from unpacking to data collection in minutes. No complex coding required.

Run-time controls let users measure and adjust the level to control the power, run timed recording intervals, adjust online filter settings, and much more.

Data courtesy of Eric Yttri lab 

Streamlined Green Fluorescence Options

iX5 – Green Photometry with Red Opto Stim

iX5 fiber photometry module with 415 nm and 465 nm LEDs and

TDT offers a version of the iX7 for performing standard green fluorescence recordings (GCaMP, GRAB sensors, dLight) plus red opto stim. The iX5 is a cost-effective option if you are not planning to record red fluorophores.

TDT offers upgrade paths to a full iX7 when you are ready to expand!

iX4 – Green Photometry

iX4 photometry module with 415 nm isosbestic excitation and 465 nm blue excitation for standard green photometry

If you just want to record green fluorescence without paying for add ons, then consider the iX4 as the best option. Stack multiple iX4s in an iCon for simple same subject multi-site photometry recordings.

TDT offers upgrade paths to a full iX7 when you are ready to expand!

Every Novel Sensor Has a Home

iX6 for Flexible Isosbestic Options

The iX6 brings the power and flexibility of TDT’s popular RZ10X processor to the iCon family. It features integrated LUX LEDs and Photosensors that plug into its front panel enabling three-color fiber photometry. Multiple iX6 devices can be employed to facilitate multi-site and multi-subject recording.

Use with TDT’s LxM optical manifolds for a turn-key system with unparalleled sensitivity and versatility. The iX6 uses TDT’s Lux line of interchangeable LEDs and Sensors for unparalleled flexibility for your calcium imaging and optogenetics experiments. Easily and cost-effectively change LEDs/wavelengths as the biological sensors in your research change over time.

Data Import and Analysis

Offline Analysis

TDT’s Synapse software data can be exported to Matlab, Python, Excel, or another analysis software of your choice. With TDT’s Fiber Photometry systems, you no longer need to worry about precisely aligning behavioral codes with neural responses. The integration of your behavioral, video, and fiber photometry data into one data structure ensures consistent synchronized timing.

Check out the these Matlab and Python examples for offline analysis code for fiber photometry.

Why TDT?

When you choose a TDT behavioral neuroscience recording system you choose legacy, innovation, and commitment. Every system is designed and assembled at our headquarters in Alachua, Florida by our long-time employees.

All images of animals include artwork (modified or otherwise) from BioRender.