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Fiber Photometry System

Conduct calcium imaging experiments with our fiber photometry processor and Synapse software.

Description

Specs & Resources

Publications

Fiber Photometry experiments have never been so easy to set up and integrate into other research projects.

The TDT Fiber Photometry System features the RZ10 acquisition processor and TDT’s Synapse software. Our Fiber Photometry System is designed specifically for researchers wanting to perform quality calcium imaging and optogenetics experiments while having access to electrophysiology and control of external behavioral devices.

Synapse and our real-time hardware architecture allow for seamless integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with minimal latency. Correlate neural responses to behavioral states or to external stimuli, such as sound, sight, and smell.

Fiber Photometry User Guide

Matlab SDK, Epoc Averaging Example, Lick Bout Epoc Filtering Example

Python SDK, Epoc Averaging Example , Lick Bout Epoc Filtering Example

Guo … Polley, et al “The Cholinergic Basal Forebrain Links Auditory Stimuli with Delayed Reinforcement to Support Learning”. Neuron Jul, 2019. doi: 10.1016/j.neuron.2019.06.024

Parker … Bruchas, et al “A Paranigral VTA Nociceptin Circuit that Constrains Motivation for Reward”. Cell Jul, 2019. doi: 10.1016/j.cell.2019.06.034

Choi … McNally, et al “Paraventricular Thalamus Controls Behavior during Motivational Conflict”. The Journal of Neuroscience Jun, 2019. doi: 10.1523/jneurosci.2480-18.2019

Feng … Li, et al “A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.02.037

Cameron … Witten, et al “Increased Cocaine Motivation Is Associated with Degraded Spatial and Temporal Representations in IL-NAc Neurons”. Neuron May, 2019. doi: 10.1016/j.neuron.2019.04.015

Mansy … Oweiss, et al “Spatial detection characteristics of a single photon fiber photometry system for imaging neural ensembles”. International IEEE/EMBS Conference on Neural Engineering, NER , 2019. doi: 10.1109/ner.2019.8717005

Bai … Knight, et al “Genetic Identification of Vagal Sensory Neurons That Control Feeding.”. Cell , 2019. doi: 10.1016/j.cell.2019.10.031

Robinson … Gradinaru, et al “Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1”. eLife , 2019. doi: 10.7554/elife.48983

Pascoli … Lüscher, et al “Stochastic synaptic plasticity underlying compulsion in a model of addiction”. Nature Dec, 2018. doi: 10.1038/s41586-018-0789-4

Reed … Britt, et al “Coordinated Reductions in Excitatory Input to the Nucleus Accumbens Underlie Food Consumption”. Neuron Sep, 2018. doi: 10.1016/j.neuron.2018.07.051

Walsh … Malenka, et al “5-HT release in nucleus accumbens rescues social deficits in mouse autism model”. Nature Aug, 2018. doi: 10.1038/s41586-018-0416-4

Saunders … Janak, et al “Dopamine neurons create Pavlovian conditioned stimuli with circuit-defined motivational properties”. Nature Neuroscience Jul, 2018. doi: 10.1038/s41593-018-0191-4

Giza … Lee, et al “The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior”. Neuron Jul, 2018. doi: 10.1016/j.neuron.2018.05.024

Beas … Penzo, et al “The locus coeruleus drives disinhibition in the midline thalamus via a dopaminergic mechanism”. Nature Neuroscience Jun, 2018. doi: 10.1038/s41593-018-0167-4

Fang … Lin, et al “A Hypothalamic Midbrain Pathway Essential for Driving Maternal Behaviors”. Neuron Apr, 2018. doi: 10.1016/j.neuron.2018.02.019

Chen … Dan, et al “A Hypothalamic Switch for REM and Non-REM Sleep”. Neuron Mar, 2018. doi: 10.1016/j.neuron.2018.02.005

Sengupta … McNally, et al “Basolateral Amygdala Neurons Maintain Aversive Emotional Salience”. The Journal of Neuroscience Mar, 2018. doi: 10.1523/jneurosci.2460-17.2017

Wang … Krauzlis, et al “Activation of Striatal Neurons Causes a Perceptual Decision Bias during Visual Change Detection in Mice”. Neuron Mar, 2018. doi: 10.1016/j.neuron.2018.01.049

Augustine … Oka, et al “Hierarchical neural architecture underlying thirst regulation”. Nature Mar, 2018. doi: 10.1038/nature25488

Hashikawa … Lin, et al “Esr1+ cells in the ventromedial hypothalamus control female aggression”. Nature Neuroscience Nov, 2017. doi: 10.1038/nn.4644

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Wells … Halassa, et al “Thalamic reticular impairment underlies attention deficit in Ptchd1Y/− mice”. Nature Mar, 2016. doi: 10.1038/nature17427

Wimmer … Halassa, et al “Thalamic control of sensory selection in divided attention”. Nature Oct, 2015. doi: 10.1038/nature15398

 

System Components

RZ10 Lux-I/O Processor

LUX LEDS AND SENSORS

Synapse Suite

PO5e Interface Card

Connect & Turn On

Connect Optics and Go with the RZ10 Lux Fiber Photometry Processor and Synapse.

TDT’s Lux Integrated Fiber Photometry (RZ10 or RZ10x) processor has built-in LEDs and photosensors for easy setup. The RZ10 processor performs real-time lock-in amplification for online demodulation of response signals.
The RZ10 also includes options to connect standalone LEDs, external LED drivers, and external photosensors for existing hardware.

Plug & Play Synapse Gizmo

Recording and light configuration is easy with the Fiber Photometry gizmo.

Synapse controls the signal parameters on the RZ10 and reads the data for viewing and storage.
The RZ10 features ultra fast digital signal processors (DSP) for optimal performance. Since Synapse will be handling your processing details, such as assigning tasks to the DSP and distributing tasks evenly, you only need to worry about higher level functions like organizing multiple experiments across numerous subjects and various lab members.

Press Record

Begin collecting and visualizing real-time fiber photometry data in no time.

Integrate synchronized video recording, behavioral study, electrophysiology, and optogenetic stimulation into your experimental paradigm with a few simple clicks. Any combination of these modalities can be run simultaneously.

 

Record from Multiple Subjects | TDT's Fiber Photometry System

 

 

Record from Multiple Subjects

The flexibility of our fiber photometry system means that multiple fiber optics can be recorded from multiple subjects. Have independent GCaMP + isosbestic control of up to two animals.

Record from Multiple Sites | TDT's Fiber Photometry System

 

Record from Multiple Sites

Dissect the dynamics of neural circuitry on a wider scale with multi-site recordings. You have independent control of three (RZ10) to six (RZ10x) LED sources with the RZ10(x) DAC outputs.

 

Record Other Neurophysiological Signals | TDT's Fiber Photometry System


Record Other Neurophysiological Signals

The RZ10 + Synapse is a flexible platform that allows for easy integration of electrophysiology and other signals into your current experiments. The RZ10(x) comes with pre-built optical ports to add a Medusa or PZ5 amplifier (RZ10x only) for doing 4 – 32 channels of ephys. Other analog signals can be captured by the remaining ADC inputs of the RZ10.

Lock-In Amplification

See many clear demodulated fluorophore responses in real-time.

Our fiber photometry system works off the principle of locked-in amplification, wherein LED light sources are driven at high frequencies (200 – 500 Hz) and low-frequency fluorescent responses are demodulated out of the raw photostream.

Technique

Lock-in amplification is a signal processing technique that uses modulation of driver signals and an orthogonal reference signal (cosine of the driver signal) to extract relevant amplitude and phase of frequency-specific responses in a complex and often noisy signal. We drive multiple signals at different frequencies and then extract only the contribution from each of those frequencies in the acquired photodetector signal – that’s how we can detect multiple fluorescent simultaneously off of a single photosource.

Benefits

Lock-in amplification has many benefits. First, it allows for high-fidelity signal detection in noisy environments. Since signals are extracted based on their frequency content, the fluorescent signal is not affected by parasitic room lighting or electronic noise. Additionally, this technique allows for detecting several signals simultaneously off the same fiber optic cable (405 isosbestic, 465 GCaMP, 560 tdTomato, etc).

Difference

Other systems use CMOS cameras or light spectroscopy to pick up fluorescent signal responses. This normally requires multiple fibers per light source and is a generally noisier technique. Our lock-in amplification makes it easy to send multiple light sources down the same fiber to detect several independent signals from the same site. No need for multiple fibers – one site, several colors, a clean signal!

Fiber Photometry Experiment Gizmos

TDT Engineers brought the RZ10 Lux Integrated Fiber Photometry Processor from concept to production and worked with our technical support team to ensure the Fiber Photometry Gizmo is relatively easy to use for any lab.

Anyone can master Fiber Photometry setup with relative ease. Connect and control all of your other S3 devices with ease using the intuitive Synapse drop-and-go gizmo functionality.

Fiber Photometry Gizmo

Data Import and Analysis

Extract the photometry signal and analyze the fluorescence output.

With TDT’s new MatlabSDK or PythonSDK, your data will be imported as a structure for straightforward handling of all streams, snips, and epoc events. There are many optional functions in the SDK, such as TDTfilter, which lets you filter data around epoc events to make peri-event response plots. You can also look at our Offline Analysis Matlab Workbooks, which are full-code examples of how to import data into Matlab and do interesting things.

There are also optional exports to ascii or other formats for analysis in Excel.

Commitment to Your Research

We understand that every experiment is dependent on your specific research. TDT service professionals work with one job in mind: to ensure that you get the help you need.

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