RZ10 Lux Integrated Processor
The RZ10 platform includes light driving/sensing capabilities for lock-in amplification fiber photometry setups. The RZ10x model has twice as many light driving/sensing ports for running multiple subjects or to add optogenetic stimulation, and includes fiber optic connections for integrated multi-channel neurophysiology.
The RZ10 Lux Integrated Processor has a single 400 MHz Sharc digital signal processor (DSP). It includes one bank of integrated Lux light drivers and photosensors. The RZ10x model (shown above) has three DSPs and two Lux banks standard. Both models are expandable to up to four DSPs.
Each Lux bank has 3 output drivers and 2 inputs. Each output port can have either a Lux LED, an M8 connector to drive an external LED, or a BNC connector to control a voltage signal. Each input port can have either a Lux PS1 photosensor, Lux PM1 power meter, or BNC connector to measure a voltage input. The components are interchangeable by the user.
The RZ10 includes one additional analog input channel. The RZ10x includes two additional analog input channels. The RZ10x fiber optic input port can acquire up to 32 channels of neurophysiological signals from a PZ amplifier at up to ~50 kHz for real-time processing synchronized to your fiber photometry data and/or optogenetic stimulation.
The RZ10 and RZ10x both feature 24-bits of digital I/O (four bits are accessible on the front panel BNC connectors) and a legacy fiber optic port to acquire neurophysiological signals from a Medusa4Z, RA8GA, or RA16PA preamplifier.
This manual provides hardware information for the RZ10. For a full application guide specific to fiber photometry using the RZ10, see the Fiber Photometry User Guide.
Power and Communication
The RZ10's Optibit optical interface connects to a PO5, PO5e, PO5c, or UZ3 computer interface card for fast and reliable data transfer from the RZ2 to the PC. Connectors on the back panel are color coded for correct wiring.
The RZ10's integrated power supply is shipped from TDT configured for the end user's regional voltage setting (110 V or 220 V). If you need to change the voltage setting:
Turn off the RZ10
Use a small flathead screwdriver to gently push the clip along the left side of the fuse plate to the right to remove it.
Remove the white AC voltage selector and rotate it until the desired voltage is displayed, then reinsert it and put the fuse plate back on.
The RZ10 is UL compliant, see the RZ10 Operator's Manual for power and safety information.
TDT's Synapse software controls the RZ10 and provides users a high level interface for device configuration.
DSP Status Lights
These LEDs report the status of the multiprocessor's individual DSPs and will be lit solid green when the corresponding DSP is installed and running. The corresponding LED will be lit dim green if the cycle usage on a DSP is 0%. If the demands on a DSP exceed 99% of its capacity on any given cycle, the corresponding LED will flash red (~1 time per second). For QZDSPs, the LED indicates levels for the core with the highest cycle usage.
Front Panel Display Screen
The front panel display screen reports detailed information about the status of the system. The top line reports the system mode, Run!, Idle, or Reset. The second line reports the user's choice of status indicators for each DSP followed by an aggregate value.
The user can cycle through the various status indicators using the Mode button to the bottom right of the display. Push and release the button to change the display or push and hold the button for one second then release to automatically cycle through each of the display options. The display screen may also report system status such as booting status (Reset).
When burning new microcode or if the firmware on the RZ is blank, the display screen will report a cycle usage of 99% and the processor status lights will flash red.
SI Fiber Optic Port
The output port labeled SI can be used with a Subject Interface, PZ5 amplifier, or iCon Behavioral Controller for up to 128 channels of electrical stimulation, 32 channels or neurophysiological recording, or behavior control.
RZ10x with serial number <2000 had a PZ port for PZ5 NeuroDigitizer support only.
Legacy Fiber Optic Port
The RZ10 has one Legacy fiber optic ports labeled Legacy Amp to acquire digitized signals from the Medusa4Z, RA16PA, or RA8GA. This port can read up to 16 channels at a maximum sampling rate of ~25 kHz.
Onboard Analog Inputs
The Onboard Lux I/O is the primary analog I/O on the RZ10. However, the RZ10 model has an extra channel of 16-bit PCM A/D input accessible through the BNC marked 'ADC4'. The RZ10x model has an additional channel marked 'ADC8'. See the Synapse Manual for information on using these inputs.
The digital I/O ports include 24 bits of programmable I/O. The digital I/O is divided into three bytes (A, B, and C) as described in the chart below. All digital I/O lines are accessed via the 25-pin connector on the front of the RZ10 and bits 0 - 3 of byte C are available through BNC connectors on the front panel labeled Digital. See RZ10 Technical Specifications for the DB25 pinout.
The digital I/O is configured in the Synapse RZn Hal.
*Note: Byte C Bits 0 - 3 are available via front panel BNCs
The data direction for the Digital I/O is also configured in the Synapse RZn Hal.
The RZ digital I/O ports have different voltage outputs and logic thresholds depending on the type. Below is a table listing the different voltage outputs and thresholds for both types.
The RZ10 has 16 LED indicators for the analog and digital I/O located directly below the display screen and DSP status LEDs.
These LEDs indicate the state of the 8 bit-addressable I/O of byte C.
These LEDs indicate the state of the four ADC and four DAC channels.
ADC channel 3 and DAC channel 4 are used internally while the RZ10 is actively running, so the ADC3 and DAC4 LEDs will flash ~1 Hz during normal operation.
UDP Ethernet Interface
The RZ UDP Ethernet interface can transfer low bandwidth data directly to or from a PC. RZ devices equipped with a UDP interface have an additional Ethernet port and RS232 serial port located on the back panel. See RZ-UDP Communications Interface for more information.
Specialized DSP/Optical Interface Boards
The RZ standard DSP boards can be replaced with specialized DSP boards which include an optical interface for communication and control of RZ compatible devices, such as the Subject Interface and RS4 Data Streamer. RZ devices equipped with one or more specialized DSP boards include an optical port for each card. The ports are located on the back panel and labeled for easy identification.
Onboard Lux I/O
The RZ10 has one bank of integrated Lux components. Each Lux bank has three built-in current driver outputs for driving integrated LEDs directly, or external LEDs connected to an M8 cable. Each Lux bank also has two input slots for photosensors and/or power meters.
The RZ10x includes a second full bank for Lux components.
The Lux components are held in place with two nylon screws. They are interchangeable, so you can swap LED colors at any time, upgrade from an external photosensor to a PS1 integrated sensor, or interface with an external device with a BNC connector. In the case of lost Lux screws, do not replace them with metal screws. Please contact TDT support for replacement screw options.
Make sure the RZ10 is powered off before changing the Lux components.
The Lux LEDs, PS1, and PM1 have an FC connector with a small key that must be aligned to the fiber optic cable. This key is in the 10 o'clock position on all TDT optical Lux components.
See the Synapse Manual for information on controlling the Lux drivers and reading the sensors.
Lux Technical Specifications
|Lux BNC Pod Output Impedance|
|PS1 and PM1 Responsivity|
RZ10 Technical Specifications
Specifications for amplifier A/D converters are found under the preamplifier's technical specifications.
BNC Channel Mapping
Please note channel numbering is printed on the face of the device to minimize mis-wiring.
DB25 Digital I/O Pinout
If using a PP24 Patch Panel, see PP24 to RZ10 Digital I/O.