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RZ10 Lux Integrated Processor

RZ10 Overview

The RZ10 platform includes light driving/sensing capabilities for lock-in amplification fiber photometry setups. The RZ10x model has twice as many light driving/sensing ports for running multiple subjects or to add optogenetic stimulation, and includes fiber optic connections for integrated multi-channel neurophysiology.

The RZ10 Lux Integrated Processor has a single 400 MHz Sharc digital signal processor (DSP). It includes one bank of integrated Lux light drivers and photosensors. The RZ10x model (shown above) has three DSPs and two Lux banks standard. Both models are expandable to up to four DSPs.

Each Lux bank has 3 output drivers and 2 inputs. Each output port can have either a Lux LED, an M8 connector to drive an external LED, or a BNC connector to control a voltage signal. Each input port can have either a Lux PS1 photosensor, Lux PM1 power meter, or BNC connector to measure a voltage input. The components are interchangeable by the user.

The RZ10 includes one additional analog input channel. The RZ10x includes two additional analog input channels. The RZ10x fiber optic input port can acquire up to 32 channels of neurophysiological signals from a PZ amplifier at up to ~50kHz for real-time processing synchronized to your fiber photometry data and/or optogenetic stimulation.

The RZ10 and RZ10x both feature 24-bits of digital I/O (four bits are accessible on the front panel BNC connectors) and a legacy fiber optic port to acquire neurophysiological signals from a Medusa4Z, RA8GA, or RA16PA preamplifier.

This manual provides hardware information for the RZ10. For a full application guide specific to fiber photometry using the RZ10, see the Fiber Photometry User Guide.

Power and Communication

The RZ10's Optibit optical interface connects to a PO5, PO5e, PO5c, or UZ3 computer interface card for fast and reliable data transfer from the RZ2 to the PC. Connectors on the back panel are color coded for correct wiring.

The RZ10's integrated power supply is shipped from TDT configured for the end user's regional voltage setting (110 V or 220 V). If you need to change the voltage setting:

  1. Turn off the RZ10

  2. Use a small flathead screwdriver to gently push the clip along the left side of the fuse plate to the right to remove it.

  3. Remove the white AC voltage selector and rotate it until the desired voltage is displayed, then reinsert it and put the fuse plate back on.

The RZ10 is UL compliant, see the RZ10 Operator's Manual for power and safety information.

Software Control

TDT's Synapse software controls the RZ10 and provides users a high level interface for device configuration.

RZ10 Features

DSP Status Lights

These LEDs report the status of the multiprocessor's individual DSPs and will be lit solid green when the corresponding DSP is installed and running. The corresponding LED will be lit dim green if the cycle usage on a DSP is 0%. If the demands on a DSP exceed 99% of its capacity on any given cycle, the corresponding LED will flash red (~1 time per second). For QZDSPs, the LED indicates levels for the core with the highest cycle usage.

Front Panel Display Screen

The front panel display screen reports detailed information about the status of the system. The top line reports the system mode, Run!, Idle, or Reset. The second line reports the user's choice of status indicators for each DSP followed by an aggregate value.

The user can cycle through the various status indicators using the Mode button to the bottom right of the display. Push and release the button to change the display or push and hold the button for one second then release to automatically cycle through each of the display options. The display screen may also report system status such as booting status (Reset).

Note

When burning new microcode or if the firmware on the RZ is blank, the display screen will report a cycle usage of 99% and the processor status lights will flash red.

Indicator Description
Cyc Cycle usage. For QZDSPs, the highest core cycle usage is shown
Bus% Percentage of internal device's bus capacity used
I/O% Percentage of data transfer capacity used
Opt Connection (sync) status of legacy amplifier

SI Fiber Optic Port

The output port labeled SI can be used with a Subject Interface, PZ5 amplifier, or iCon Behavioral Controller for up to 128 channels of electrical stimulation, 32 channels or neurophysiological recording, or behavior control.

Note

RZ10x with serial number < 2000 had a PZ port for PZ5 NeuroDigitizer support only.

Legacy Fiber Optic Port

The RZ10 has one Legacy fiber optic ports labeled Legacy Amp to acquire digitized signals from the Medusa4Z, RA16PA, or RA8GA. This port can read up to 16 channels at a maximum sampling rate of ~25 kHz.

Onboard Analog Inputs

The Onboard Lux I/O is the primary analog I/O on the RZ10. However, the RZ10 model has an extra channel of 16-bit PCM A/D input accessible through the BNC marked 'ADC4'. The RZ10x model has an additional channel marked 'ADC8'. See the Synapse Manual for information on using these inputs.

Digital I/O

The digital I/O ports include 24 bits of programmable I/O. The digital I/O is divided into three bytes (A, B, and C) as described in the chart below. All digital I/O lines are accessed via the 25-pin connector on the front of the RZ10 and bits 0 - 3 of byte C are available through BNC connectors on the front panel labeled Digital. See RZ10 Technical Specifications for the DB25 pinout.

The digital I/O is configured in the Synapse RZn Hal.

Digital I/O Description DB25 BNCs Notes
Byte A bits 0 - 7 Yes No byte addressable
Byte B bits 0 - 7 Yes No byte addressable
Byte C bits 0 - 7 Yes Yes* bit addressable

*Note: Byte C Bits 0 - 3 are available via front panel BNCs

The data direction for the Digital I/O is also configured in the Synapse RZn Hal.

The RZ digital I/O ports have different voltage outputs and logic thresholds depending on the type. Below is a table listing the different voltage outputs and thresholds for both types.

Digital I/O Type Output High Output Low Input High Input Low
Port A & Port B 5 V 0 V >= 2.5 V 0 - 2.45 V
Port C 3.3 V 0 V >= 1.5 V 0 - 1.4 V

LED Indicators

The RZ10 has 16 LED indicators for the analog and digital I/O located directly below the display screen and DSP status LEDs.

Digital I/O

These LEDs indicate the state of the 8 bit-addressable I/O of byte C.

Light Pattern Description
Dim Green Bit is configured for output and is currently a logical low (0)
Solid Green Bit is configured for output and is currently a logical high (1)
Dim Red Bit is configured for input and is currently a logical low (0)
Solid Red Bit is configured for input and is currently a logical high (1)

Analog I/O

These LEDs indicate the state of the four ADC and four DAC channels.

Light Pattern Description
Off Analog I/O channel signal voltage is less than ± 100 mV
Dim Green Analog I/O channel signal voltage is less than ± 5 V
Solid Green Analog I/O channel signal voltage is between ± 5 V to ± 9 V
Solid Red Analog I/O channel clip warning (voltage greater than ± 9 V)

Note

ADC channel 3 and DAC channel 4 are used internally while the RZ10 is actively running, so the ADC3 and DAC4 LEDs will flash ~1Hz during normal operation.

UDP Ethernet Interface

The RZ UDP Ethernet interface can transfer low bandwidth data directly to or from a PC. RZ devices equipped with a UDP interface have an additional Ethernet port and RS232 serial port located on the back panel. See RZ-UDP Communications Interface for more information.

Specialized DSP/Optical Interface Boards

The RZ standard DSP boards can be replaced with specialized DSP boards which include an optical interface for communication and control of RZ compatible devices, such as the Subject Interface and RS4 Data Streamer. RZ devices equipped with one or more specialized DSP boards include an optical port for each card. The ports are located on the back panel and labeled for easy identification.

DSP Supported Peripheral
RZDSP-I IZ2 Stimulators
RZDSP-M Subject Interface, PZ5 amplifier, iCon Behavioral Controller
RZDSP-P PZ amplifier. It can be used to expand the number of channels that can be acquired on any RZ processor.
RZDSP-S RS4 Data Streamer, allowing the RZ device to stream data directly to the RS4's storage arrays.
RZDSP-U PO8e interface card, allowing the RZ device to stream data directly to client software.
RZDSP-V RV2 Video Tracking System
QZDSP_OPT Supports all of the above devices.

Onboard Lux I/O

The RZ10 has one bank of integrated Lux components. Each Lux bank has three built-in current driver outputs for driving integrated LEDs directly, or external LEDs connected to an M8 cable. Each Lux bank also has two input slots for photosensors and/or power meters.

The RZ10x includes a second full bank for Lux components.

The Lux components are held in place with two nylon screws. They are interchangeable, so you can swap LED colors at any time, upgrade from an external photosensor to a PS1 integrated sensor, or interface with an external device with a BNC connector. In the case of lost Lux screws, do not replace them with metal screws. Please contact TDT support for replacement screw options.

Important

Make sure the RZ10 is powered off before changing the Lux components.

Lux Pod Description
Each Lux LED pod is colored with the closest visible color to the wavelength of light it emits. The Lux LEDs can occupy the left three slots of each Lux bank. The 465nm Lux LED is shown at left.
The PS1 photosensor measures the fluorescence response from the subject. It can occupy the right two slots of each Lux bank.
The PM1 Power Meter measures the LED power received by the subject. It has built-in fluorescing material so it can also mimic a subject response for full end-to-end system testing. The PM1 can only occupy the slot on the far right of each Lux bank. The PM1 Power Meter measures the LED power received by the subject. It has built-in fluorescing material so it can also mimic a subject response for full end-to-end system testing. The PM1 can only occupy the slot on the far right of each Lux bank.
The M8 connector is the default placeholder for the left three slots if LEDs aren't installed. It is used to drive an external LED. This can be a Lux LED in an external heat sink, or a third-party LED.
The BNC connector can be swapped into any location on the Lux bank to connect to third party devices. It is the default placeholder for the right two slots if PS1/PM1s aren't installed.

Important

The Lux LEDs, PS1, and PM1 have an FC connector with a small key that must be aligned to the fiber optic cable. This key is in the 10 o'clock position on all TDT optical Lux components.

See the Synapse Manual for information on controlling the Lux drivers and reading the sensors.

Lux Technical Specifications

LEDs
Available Wavelengths 385 nm, 405 nm, 415 nm, 450 nm, 465 nm,
500 nm, 530 nm, 560 nm, 590 nm, 615 nm,
635 nm, 850 nm, 940 nm, 5K
Current Range 2 mA - 1000 mA
PS1
Bandwidth DC - 700 Hz
Wavelength Range 320 nm - 1100 nm
Gain 1e10
PM1
Bandwidth DC - 3000 Hz
Wavelength Range 320 nm - 1100 nm
Gain 6.5e4
BNC Output Pod
Output Channels RZ10: up to 3 channels, 16-bit PCM
RZ10x: up to 6 channels, 16-bit PCM
Sample Rate Up to 48828.125 Hz
Frequency Response DC - 0.44*Fs (Fs = sample rate)
Voltage Out ± 10.0 V, 6 mA max load
S/N (typical) 82 dB (20 Hz - 20 kHz at 9.9 V)
Output Impedance See below
BNC Input Pod
Input Channels RZ10: up to 2 channels, 16-bit PCM
RZ10x: up to 4 channels, 16-bit PCM
Sample Rate Up to 48828.125 Hz
Frequency Response DC - 7.5 kHz (2nd order, 12 dB per octave)
Voltage In ± 10.0 V
S/N (typical) 82 dB (20 Hz - 20 kHz at 9.9 V)
Input Impedance 10 kOhms (impedance of input connection
will appear to be ~400 Ohm higher)

Lux BNC Pod Output Impedance

PS1 and PM1 Responsivity

RZ10 Technical Specifications

Note

Specifications for amplifier A/D converters are found under the preamplifier's technical specifications.

DSP Up to four standard DSPs and/or quad-core (QZDSP)
DSP: 400 MHz DSPs, 2.4 GFLOPS peak per DSP
QZDSP: Four 400 MHz DSPs, 2.4 GFLOPS per core
Memory 64 MB SDRAM per standard DSP
256 MB DDR2 RAM per core, four cores per QZDSP
Digital I/O 8 programmable bits: 3.3 V (5.0 V max input), 25 mA max load
2 programmable bytes (16 bits): 5.0 V (6.0 V max input), 35 mA max load
A/D 1 channel, 16-bit PCM
Sample Rate Up to 48828.125 Hz
Frequency Response DC - 7.5 kHz (3 dB corner, 2nd order, 12 dB per octave)
Voltage In ± 10.0 V
S/N (typical) 82 dB (20 Hz - 20 kHz at 9.9 V)
Input Impedance 10 kOhms
Fiber Optic Ports
SI RZ10x (serial >= 2000): One connection for Subject Interface, PZ5, or iCon
PZ RZ10x (serial < 2000): One connection for PZ5 NeuroDigitizer
Legacy Amp (Medusa) 16-channel input, up to 24414.0625 Hz

BNC Channel Mapping

Please note channel numbering is printed on the face of the device to minimize mis-wiring.

Maps To:
Ch 4 Analog In

Ch 8 Analog In (RZ10x only)
Maps To:
Port C Bits 0-3 Digital I/O

DB25 Digital I/O Pinout

Pin Name Description Pin Name Description
1 C0 Port C Bit Addressable 14 C1 Port C Bit Addressable
2 C2 15 C3
3 C4 16 C5
4 C6 17 C7
5 GND Digital I/O Ground 18 A0 Port A Word Addressable
6 A1 Port A Word Addressable 19 A2
7 A3 20 A4
8 A5 21 A6
9 A7 22 B0 Port B Word Addressable
10 B1 Port B Word Addressable 23 B2
11 B3 24 B4
12 B5 25 B6
13 B7

If using a PP24 Patch Panel, see PP24 to RZ10 Digital I/O.