Skip to content

Lab Rat Hardware

Analog Headstage Interface

A DB26 connector for interfacing the AC16LR or the SB4 to the Lab Rat for up to 16 channels of neural signal acquisition.

DB26 Neural Input - Shared Reference Mode

Pin Name Description Pin Name Description
1 A1 Analog Input Channels 14 V+ Positive Voltage (+2.5V)
2 A2 15 GND Ground
3 A3 16 GND Ground
4 A4 17 V- Negative Voltage (-2.5V)
5 Ref Reference 18 HSD Headstage Detect
6 HSD Headstage Detect 19 HSD
7 A5 Analog Input Channels 20 A6 Analog Input Channels
8 A7 21 A8
9 A9 22 A10
10 A11 23 A12
11 A13 24 A14
12 A15 25 A16
13 AltRef Not Used 26 NC Not Used

DB26 Neural Input - Differential Mode


There are 8 (+) channels and 8 (-) channels per DB26 connector.

Pin Name Description Pin Name Description
1 A1(+) Analog Input Channels 14 V+ Positive Voltage (+2.5V)
2 A1(-) 15 GND Ground
3 A2(+) 16 GND Ground
4 A2(-) 17 V- Negative Voltage (-2.5V)
5 NC Not Used 18 HSD Headstage Detect
6 HSD Headstage Detect 19 HSD
7 A3(+) Analog Input Channels 20 A3(-) Differential Input Channels
8 A4(+) 21 A4(-)
9 A5(+) 22 A5(-)
10 A6(+) 23 A6(-)
11 A7(+) 24 A7(-)
12 A8(+) 25 A8(-)
13 GND Ground 26 NC Not Used


Contact TDT technical support (+1.386.462.9622 or before attempting to make any custom connections.

Digital Headstage Interface

A 12-pin Omnetics nano connector used for interfacing with an Intan-based digital headstage, such as TDT's ZD32 Digital ZIF-Clip® headstage. The interface supports up to 32 channels of digital data acquisition.

USB-C 3.0 Connector

A reversible USB connector for communication between the Lab Rat and a Windows-based computer. Use the provided C-to-A adapter if your computer/laptop doesn't have a C-style USB connector. The laptop or computer must support USB 3.0.

DB25 Multi I/O Port

This port provides access to 16 bits of digital I/O, two ±3 V analog inputs, two ±3 V analog outputs, and power. Pinouts are shown looking into the connector.

Pin Name Description Pin Name Description
1 C0 Port C Bit Addressable 14 C1 Port C Bit Addressable
2 C2 Digital I/O 15 C3 Digital I/O
3 C4 Bits 0, 2, 4, 6 16 C5 Bits 1, 3, 5, 7
4 C6 +3.3V, 10mA max per bit 17 C6 +3.3V, 10mA max per bit
5 GND Ground 18 A0 Port A Word Addressable
6 A1 Port A Word Addressable 19 A2 Digital I/O
7 A3 Digital I/O 20 A4 Bits 0, 2, 4, 6
8 A5 Bits 1, 3, 5, 7 21 A6 +3.3V, 10mA max per bit
9 A7 +3.3V, 10mA max per bit 22 ADC1 Analog Input Channel 1
±3V, 20mA max
10 ADC2 Analog Input Channel 2
±3V, 20mA max
23 VBUS +5V, 150mA max
11 VCC +3.3V, 250mA max 24 DAC1 Analog Output Channel 1
±3V, 20mA max
12 DAC2 Analog Output Channel 2
±3V, 20mA max
25 V+ +3.3V, 150mA max
13 V- -3.3V, 150mA max

Bit 0 and Bit 1 BNC Ports

Bit0 and Bit1 on the Lab Rat interface module are BNC connections that map to Bits C0 and C1 on the Lab Rat. These connections are ideal for synchronizing the Lab Rat with BNC-based TTL triggers to and from external hardware. These can be configured in the Lab Rat object in Synapse Lite software. When performing TTL communications over BNC cables, be sure to use a shielded and as-short-as-possible cable for maximum signal integrity.

Stereo A/D and D/A Ports

Each of the ADC / DAC ports are ⅛" stereo jacks for two-channel analog signals for interacting with external hardware with up to ±3V dynamic range. The speaker on the top of the Lab Rat is connected to the first D/A channel. The level knob on the Lab Rat interface module controls the speaker volume. The ADC input impedance is 200kΩ.


Do not exceed ±5V on the ADC inputs

Status LED

The LED on the Lab Rat interface module indicates the run-time status of the device. Listed below are the various indicator states of the status LED:

Green LED

Solid - device is ready to run, system is idle.

Flashing - device is running and acquiring data.


Flashing - device is running but there is a synchronization error with the computer. This happens if Corpus is not reading data fast enough, which means there is some other process on your computer that is consuming too many resources. There will also be a data acquisition error in Synapse Lite. If the error does not resolve itself, check for other running processes that might be interfering with Corpus and restart your recording in Synapse.

Power External Devices

You can use the VCC and VBUS pins on the Multi I/O Port to power external devices, such as sensors, relays, servos, and more. Be sure to check the specs of your external device and compare it to the allowed power draw listed in the LR10 DB25 Multi I/O Port pinout on the previous page.